Feasibility of genomic profiling with next-generation sequencing using specimens obtained by image-guided percutaneous needle biopsy

Miyuki  Sone; Yasuaki  Arai; Shunsuke  Sugawara; Takatoshi  Kubo; Chihiro  Itou; Tetsuya  Hasegawa; Noriyuki  Umakoshi; Noboru  Yamamoto; Kumiko  Sunami; Nobuyoshi  Hiraoka; Takashi  Kubo

doi:10.1080/03009734.2019.1607635

Miyuki Sone Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Yasuaki Arai Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Shunsuke Sugawara Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Takatoshi Kubo Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Chihiro Itou Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Tetsuya Hasegawa Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Noriyuki Umakoshi Department of Diagnostic Radiology, National Cancer Center Hospital, Tokyo, Japan
Noboru Yamamoto Department of Experimental Therapeutics, National Cancer Center Hospital, Tokyo, Japan
Kumiko Sunami Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan
Nobuyoshi Hiraoka Department of Pathology and Clinical Laboratories, National Cancer Center Hospital, Tokyo, Japan
Takashi Kubo Division of Translational Genomics, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo, Japan

DOI: https://doi.org/10.1080/03009734.2019.1607635

Keywords: Biopsy, genomic analysis, needle biopsy, nextgeneration sequencing

Abstract

Aims: The demand for specimen collection for genomic profiling is rapidly increasing in the era of personalized medicine. Percutaneous needle biopsy is recognized as minimally invasive, but the feasibility of comprehensive genomic analysis using next-generation sequencing (NGS) is not yet clear. The purpose of this study was to evaluate the feasibility of genomic analysis using NGS with specimens obtained by image-guided percutaneous needle biopsy with 18-G needles.

Patients and methods: Forty-eight patients who participated in a clinical study of genomic profiling with NGS with the specimen obtained by image-guided needle biopsy were included. All biopsies were performed under local anesthesia, with imaging guidance, using an 18-G cutting needle. A retrospective chart review was performed to determine the rate of successful genomic analysis, technical success rate of biopsy procedure, adverse events, rate of success in pathological diagnosis, and cause of failed genomic analysis.

Results: The success rate of genomic analysis was 79.2% (38/48). The causes of failure were unprocessed for DNA extraction due to insufficient specimen volume (6/10), insufficient DNA volume (2/10), and deteriorated DNA quality (2/10). The rate of successful genomic analysis excluding NGS analysis that failed for reasons unrelated to the biopsy procedures was 95.2% (40/42). Technical success of biopsy was achieved in all patients without severe adverse events. The rate of success in the pathological diagnosis was 97.9% (47/48).

Conclusions: Image-guided needle biopsy specimens using an 18-G cutting needle yielded a successful NGS genomic analysis rate with no severe adverse events and could be an adoptable method for tissue sampling for NGS.

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