Serum concentrations of Thymidine kinase 1 measured using a novel antibody-based assay in patients with Hodgkin Lymphoma

  • Johan Mattsson Ulfstedt Experimental and Clinical Oncology, Department of Immunology, Genetics and Pathology; Uppsala University, Uppsala, Sweden https://orcid.org/0000-0003-0682-7394
  • Per Venge Department of Medical Sciences, Uppsala University, Uppsala, Sweden; and Diagnostics Development, Uppsala, Sweden https://orcid.org/0000-0001-5863-790X
  • Sofia Holmgren Diagnostics Development, Uppsala, Sweden
  • Gunilla Enblad Experimental and Clinical Oncology, Department of Immunology, Genetics and Pathology; Uppsala University, Uppsala, Sweden https://orcid.org/0000-0002-0594-724X
  • Staffan Eriksson Department of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden; and AroCell AB, Uppsala, Sweden
  • Daniel Molin Experimental and Clinical Oncology, Department of Immunology, Genetics and Pathology; Uppsala University, Uppsala, Sweden
Keywords: Hodgkin lymphoma, TK1, Thymidine kinase, prognostic markers, chemotherapy

Abstract

Background: Thymidine kinase 1 (TK1) is an intracellular protein associated with DNA synthesis, expressed during the G1 phase and remained elevated through the M phase, with a potential as a biomarker for cell proliferation. In this study, we explore the possible use of TK1 in Hodgkin lymphoma (HL).

Methods: Serum concentrations of TK1 (S-TK1) were measured in 46 newly diagnosed HL patients using prospectively collected biobanked serum samples. The samples were analyzed using a novel antibody-based TK1 immunosorbent assay (ELISA).

Results: The concentrations of S-TK1 were elevated in HL patients compared with healthy controls (median 0.32 μg/L vs. 0.24 μg/L, P = 0.003). A further increase in S-TK1 was observed during the treatment. The S-TK1 concentrations were higher in patients with advanced stage disease, low B-Hb, elevated P-LD and in those with B-symptoms. A high ESR correlated with low S-TK1.

Conclusions: The study results suggest that S-TK1, measured using a novel antibody-based assay, has the potential to be a biomarker in HL. However, while S-TK1 levels are elevated at baseline compared with healthy controls, a limited number of patients and comparatively short follow-up time render reliable conclusions difficult.

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Published
2021-08-20
How to Cite
Mattsson Ulfstedt, J., Venge, P., Holmgren, S., Enblad, G., Eriksson, S., & Molin, D. (2021). Serum concentrations of Thymidine kinase 1 measured using a novel antibody-based assay in patients with Hodgkin Lymphoma. Upsala Journal of Medical Sciences, 126(1). https://doi.org/10.48101/ujms.v126.6119
Section
Original Articles