Determination of Sialic Acid Residues in Transferrin by Oxidative-Reductive Immunoassay

Erik  Cervén; Gunnar  Ronquist

doi:10.3109/03009738609178487

Determination of Sialic Acid Residues in Transferrin by Oxidative-Reductive Immunoassay

Erik Cervén Institute of Medical and Physiological Chemistry, Biomedical Center and Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden
Gunnar Ronquist Institute of Medical and Physiological Chemistry, Biomedical Center and Department of Clinical Chemistry, University Hospital, University of Uppsala, Sweden

DOI: https://doi.org/10.3109/03009738609178487

Abstract

A method is described for the determination of sialic acid residues in glycoproteins displaying microheterogeneity in the sugar residue. The new method is based on combining an oxidative-reductive step with binding of the glycoprotein to an immunoadsorbent. After a mild oxidation with sodium metaperiodate the sugar is reduced with labeled sodiumborotritide. The chemical modification of the sugar residues does not seem to impair the binding of glycoprotein to the immunoadsorbent. The procedure, which has been elaborated for human transferrin, can be carried out in the presence of other substances in body fluids.

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Published

1986-03-01

How to Cite

Cervén E., & Ronquist G. (1986). Determination of Sialic Acid Residues in Transferrin by Oxidative-Reductive Immunoassay. Upsala Journal of Medical Sciences, 91(1), 17-27. https://doi.org/10.3109/03009738609178487

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Issue

Vol. 91 No. 1 (1986): Upsala J Med Sci

Section

Original Articles

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