A Simplified Method for Determination of Pyridoxal Phosphate in Biological Samples

Arne  Hamfelt

doi:10.3109/03009738609178496

A Simplified Method for Determination of Pyridoxal Phosphate in Biological Samples

Arne Hamfelt Department of Clinical Chemistry, Central Hospital Sundsvall, Sundsvall, Sweden

DOI: https://doi.org/10.3109/03009738609178496

Abstract

A new method for the determination of pyridoxal phosphate in plasma and in cerebrospinal fluid is described. It is based on the generally accepted apotyrosine decarboxylase method, but, in stead of following the decarboxylation rate using ¹⁴C- och ³H-labelled tyrosine, a high performance liquid chromatography (HPLC) method for separation of tyrosine and tyramine is used.

This procedure is recommended as it is less time consuming and avoids utilizing radioactive material.

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Published

1986-03-01

How to Cite

Hamfelt A. (1986). A Simplified Method for Determination of Pyridoxal Phosphate in Biological Samples. Upsala Journal of Medical Sciences, 91(1), 105-109. https://doi.org/10.3109/03009738609178496

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Issue

Vol. 91 No. 1 (1986): Upsala J Med Sci

Section

Original Articles

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