@article{Klint_Lejonklou_Karimullina_Rönn_Lind_Lind_Brittebo_2016, title={Low-dose exposure to bisphenol A in combination with fructose increases expression of genes regulating angiogenesis and vascular tone in juvenile Fischer 344 rat cardiac tissue}, volume={122}, url={https://ujms.net/index.php/ujms/article/view/6252}, DOI={10.1080/03009734.2016.1225870}, abstractNote={<p><strong>Objectives:</strong>&nbsp;Epidemiological studies report associations between exposure to the high-volume chemical and endocrine disruptor bisphenol A (BPA) and cardiovascular disorders, but there is a lack of experimental studies addressing the mechanisms of action of BPA on the cardiovascular system. In the present study, effects on markers for cardiovascular function of exposure to BPA and fructose&nbsp;<em>in vivo</em>&nbsp;in rat cardiac tissues, and of BPA exposure in human cardiomyocytes&nbsp;<em>in vitro</em>, were investigated.</p> <p><strong>Materials:</strong>&nbsp;Juvenile female Fischer 344 rats were exposed to 5, 50, and 500 μg BPA/kg bodyweight/day in their drinking water from 5 to 15 weeks of age, in combination with 5% fructose. Further, cultured human cardiomyocytes were exposed to 10 nM BPA to 1 × 10<sup>4</sup>&nbsp;nM BPA for six hours. Expression of markers for cardiovascular function and BPA target receptors was investigated using qRT-PCR.</p> <p><strong>Results:</strong>&nbsp;Exposure to 5 μg BPA/kg bodyweight/day plus fructose increased mRNA expression of&nbsp;<em>Vegf</em>,&nbsp;<em>Vegfr2</em>,&nbsp;<em>eNos</em>, and&nbsp;<em>Ace1</em>&nbsp;in rat heart. Exposure of human cardiomyocytes to 1 × 10<sup>4</sup>&nbsp;nM BPA increased mRNA expression of&nbsp;<em>eNOS</em>&nbsp;and&nbsp;<em>ACE1</em>, as well as&nbsp;<em>IL-8</em>&nbsp;and&nbsp;<em>NFκβ</em>&nbsp;known to regulate inflammatory response.</p> <p><strong>Conclusions:</strong>. Low-dose exposure of juvenile rats to BPA and fructose induced up-regulation of expression of genes controlling angiogenesis and vascular tone in cardiac tissues. The observed effects of BPA in rat heart were in line with our present and previous studies of BPA in human endothelial cells and cardiomyocytes. These findings may aid in understanding the mechanisms of the association between BPA exposure and cardiovascular disorders reported in epidemiological studies.</p&gt;}, number={1}, journal={Upsala Journal of Medical Sciences}, author={Klint Helén and Lejonklou Margareta H. and Karimullina Elina and Rönn Monika and Lind Lars and Lind P. Monica and Brittebo Eva}, year={2016}, month={Sep.}, pages={20–27} }