@article{Saaed_Chiggiato_Webb_Rehnberg_Rubio_Befrits_Hellström_2021, title={Elevated gaseous luminal nitric oxide and circulating IL-8 as features of <em>Helicobacter pylori</em>-induced gastric inflammation}, volume={126}, url={https://ujms.net/index.php/ujms/article/view/8116}, DOI={10.48101/ujms.v126.8116}, abstractNote={<p><strong>Background</strong>: Gastric nitric oxide (NO) production in response to&nbsp;<em>Helicobacter pylori</em>&nbsp;via inducible nitric oxide synthase (iNOS) is suggested as a biomarker of inflammation and cytotoxicity. The aim of this study was to investigate relationships between gastric [NO], immunological biomarkers and histopathology.</p> <p><strong>Materials and methods</strong>: Esophagogastroduodenoscopy was done in 96 dyspepsia patients. Luminal [NO] was measured by chemiluminescence. Biopsies were taken from gastric antrum and corpus for culture and histopathology.&nbsp;<em>H. pylori</em>&nbsp;IgG was detected by immunoblot assay. Biobanked plasma from 76 dyspepsia patients (11&nbsp;<em>H. pylori</em>&nbsp;positives) was analyzed for 39 cytokines by multiplexed ELISA.</p> <p><strong>Results</strong>:&nbsp;<em>H. pylori</em>-positive patients had higher [NO] (336 ± 26 ppb, mean ± 95% CI,&nbsp;<em>n</em>&nbsp;= 77) than&nbsp;<em>H. pylori</em>-negative patients (128 ± 47 ppb,&nbsp;<em>n</em>&nbsp;= 19) (<em>P</em>&nbsp;&lt; 0.0001). Histopathological changes were found in 99% of&nbsp;<em>H. pylori</em>-positive and 37% of&nbsp;<em>H. pylori</em>-negative patients. Histopathological concordance was 78–100% between corpus and antrum. Correlations were found between gastric [NO] and severity of acute, but not chronic, inflammation. Plasma IL-8 (increased in&nbsp;<em>H. pylori</em>&nbsp;positives) had greatest difference between positive and negative groups, with eotaxin, MIP-1β, MCP-4, VEGF-A, and VEGF-C also higher (<em>P</em>&nbsp;&lt; 0.004 to&nbsp;<em>P</em>&nbsp;&lt; 0.032). Diagnostic odds ratios using 75% cut-off concentration were 7.53 for IL-8, 1.15 for CRP, and 2.88 for gastric NO.</p> <p><strong>Conclusions</strong>: Of the parameters tested, increased gastric [NO] and circulating IL-8 align most consistently and selectively in&nbsp;<em>H. pylori</em>-infected patients. Severity of mucosal inflammatory changes is proportional to luminal [NO], which might be tied to IL-8 production. It is proposed that IL-8 be further investigated as a blood biomarker of treatment outcomes.</p&gt;}, number={1}, journal={Upsala Journal of Medical Sciences}, author={Saaed Hiwa K. and Chiggiato Lisa and Webb Dominic-Luc and Rehnberg Ann-Sofie and Rubio Carlos A. and Befrits Ragnar and Hellström Per M.}, year={2021}, month={Oct.} }