Effect of renal sympathetic nerve on adrenergically and angiotensin II-induced renal vasoconstriction in normal Wistar-Kyoto rats

  • Mohammed H. Abdulla School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Munavvar A. Sattar School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Nor A. Abdullah Department of Pharmacology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia
  • Abdul Hye Khan Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, USA
  • Kolla R. L. Anand Swarup School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Hassaan A. Rathore School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Raisa N. Kazi School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Fathihah Basri School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia
  • Edward J. Johns Department of Physiology, Aras Windle, University College Cork, College Road, Cork, Ireland
Keywords: α1-Adrenoceptors, carvedilol, losartan, renal haemodynamics, Wistar-Kyoto rats

Abstract

Background. This study examined the effect of renal sympathetic innervation on adrenergically and angiotensin II (Ang II)-induced renal vasoconstriction in Wistar-Kyoto (WKY) rats.

Methods. Forty-eight WKY rats were treated with either losartan (10 mg/kg/day p.o.) or carvedilol (5 mg/kg/day p.o.) or a combination of them (10 mg/kg/day + 5 mg/kg/day p.o.) for 7 days. On day 8, the rats were anaesthetized, and renal vasoconstrictor experiments were carried out. A group of rats was subjected to acute unilateral renal denervation during the acute study. Changes in the renal vasoconstrictor responses were determined in terms of reductions in renal blood flow caused by Ang II, noradrenaline (NA), and methoxamine (ME).

Results. In normal animals, losartan decreased (P < 0.05) the renal vasoconstrictor response to Ang II but not to NA or ME. Carvedilol treatment, however, blunted (P < 0.05) the renal vasoconstrictor responses to Ang II and adrenergic agonists. Combination of losartan and carvedilol blunted (P < 0.05) the renal vasoconstrictor response to Ang II but augmented the responses to NA and ME (all P < 0.05). Interestingly, when denervated rats were treated with the same combination, there was a reduction (P < 0.05) in the renal vasoconstrictor responses to Ang II and adrenergic agonists.

Conclusions. Data suggest that the renal sympathetic nerve contributes to adrenergic agonist-mediated renal vasoconstrictions in normal rats. The data further indicate an interactive relationship between renin-angiotensin and sympathetic nervous systems in modulating adrenergically and Ang II-induced renal vasoconstriction in WKY rats.

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Published
2010-11-04
How to Cite
Abdulla, M. H., Sattar, M. A., Abdullah, N. A., Khan, A. H., Anand Swarup, K. R. L., Rathore, H. A., Kazi, R. N., Basri, F., & Johns, E. J. (2010). Effect of renal sympathetic nerve on adrenergically and angiotensin II-induced renal vasoconstriction in normal Wistar-Kyoto rats. Upsala Journal of Medical Sciences, 116(1), 18–25. https://doi.org/10.3109/03009734.2010.526723
Section
Original Articles