Lipid Metabolism of Isolated Mouse Pancreatic Islets Maintained in Culture at Different Glucose Concentrations

  • Christian Berne
  • Arne Andersson

Abstract

The lipid metabolism of isolated mouse pancreatic islets, which had been cultured for one week at 3.3 or 16.7 mM glucose, was examined in short-term experiments performed at the end of the culture period. It was found that: a) highglucose cultured islets oxidized palmitate at a higher rate: and b) both groups of cultured islets increased the rate of incorporation of (U-14C)-glucose into triacylglycerols and phospholipids in response to an acute glucose challenge. This latter effect was, however, more pronounced after culture at a high glucose concentration. The present data suggest that long-term exposure to high concentrations of glucose leads to a general increase of islet oxidative metabolism. Moreover, high glucose culture seems to induce an increased conversion of carbohydrates into triacylglycerols and membrane phospholipids.

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Published
1981-03-01
How to Cite
Berne C., & Andersson A. (1981). Lipid Metabolism of Isolated Mouse Pancreatic Islets Maintained in Culture at Different Glucose Concentrations. Upsala Journal of Medical Sciences, 86(1), 55-61. https://doi.org/10.3109/03009738109179210
Section
Original Articles

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